in reply to Key value pair in hash inside while loop

When I run your code on the data sample, I get
A 25 C 25 G 25 U 25

What do you mean by "value as zero"? What output do you expect?

لսႽ† ᥲᥒ⚪⟊Ⴙᘓᖇ Ꮅᘓᖇ⎱ Ⴙᥲ𝇋ƙᘓᖇ