That error message seems to contain smart quotes --- that can't work (why did you type or copy/paste smart quotes)

"Can't open perl script "“C:\inetpub\wwwroot\test.pl": Invalid argument ".

""""""""" and “““““““““““ and ””””””””””” are different and windows doesn't like all of them

See also
http://docs.activestate.com/activeperl/5.18/faq/Windows/ActivePerl-Winfaq6.html#microsoft_web_servers
ActivePerl and IIS discussion | ActiveState Community Site