Hi, Thanks for the reply

The blastn is not related to the hash,the hash contains data from sequencing reads, the files for the blastn are very small

The hash is being used constantly, so that why it needs be a hash, other methods like databases or writing to files, is too slow

And if change the blastn system call to system("echo Hello"), I get the same problem, so it's not related to the bastn

That RAM disk is maybe to complicated for users of the tool, mostly biologists who I need to explain what a terminal is :)