Again, please read Building Regex Alternations Dynamically for a very effective approach to this problem.

my $file1 = \<<"END1";
he/is/man/reg[30]
what/goes/on/reg[32]
i_am_a_man
you_are_reg[60]
END1

my $file2 = \<<"END2";
/is/man/reg[30]
are_reg[60] 
try/to/do/reg[65]
you/reg[31]
END2

 
  
   open(my $f3, ">", "C:/Users/Siddharth/Desktop/two.txt")
    or die "Can't open < input.txt: $!";
open  my $h2, '<', $file2 or die "cannot open file2";
my %map = <$h2>;

my ($regex) = map { qr/$_/ }          # 2.
    join '|', map {quotemeta}
    close $h2;


open  my $h1, '<', $file1 or die "cannot open file1";
my @a1= <$h1>;


 

#print grep {$_ =~ $regex} @a1;
 
print $f3  grep {$_ !~ $regex} @a1;
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2020-06-21 Athanasius added code tags.

i have written above as modified version of previous code(partial mismatching)with the help of reference given by you all as a link,still i am not getting correct output. all lines of file1 are coming as output. whereas output should come as 1. what goes on reg32 2. i_am_a_man please help