Out of interest, what sort of audio are you talking about, voice/mic input? The reason I ask is that cross browser, low latency audio workflows I think may cause issues, especially if it has to work cross browser on 'consumer' hardware. Regardless, that's going to be a client side thing, JavaScript(one example)/Web Audio, sending the resultant audio to a perl backend, for which I'd suggest a Mojolicious::Lite/Mojolicious framework, depending on your needs.