If I understood the question, something like the below should work.

#!/bin/perl -w
use strict;

open TMP, "input.txt" || die ("could not open the input file \n");
my (%hash, @columns);
for (<TMP>) {
  chomp;
  @columns = split ;
  next unless ($columns[1] && $columns[2]);
  if (not exists $hash{$columns[1].$columns[2]}) {
    $hash{$columns[1].$columns[2]}=1;
    print $_."\n" ;
  }
}
close TMP;
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