As swl suggested, you can read/write data as a range.

#!perl
use strict; 
use Win32::OLE;
use Win32::OLE::Const "Microsoft Excel";
use Data::Dump 'pp';

$Win32::OLE::Warn = 3;

my $Excel =Win32::OLE->new('Excel.Application');
$Excel->{Visible} = 1;

my $Book = $Excel->Workbooks->Open('c:/temp/Book1.xlsx');
my $Sheet = $Book->Worksheets("Sheet1");

my $begincol = 2; my $beginrow = 6; # B6
my $endcol   = 4; my $endrow   = 10;# D10
my $c1 = $Sheet->Cells($beginrow,$begincol);
my $c2 = $Sheet->Cells($endrow,$endcol);
my $data = $Sheet->Range($c1,$c2)->Value;
pp $data;

$Sheet->Range("A16:C20")->{'Value'} = $data;
$Excel->Quit();
undef $Excel;
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