Yes you would. When you assign a read from a filehandle in a scalar context the file pointer will be incremented to the next line according to the $/ variable e.g

while(<FH>) {
    my $nextline = <FH>;
}
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What happens here is that the first line of <FH> is assigned to $_ and the filehandle will point to the second line. When $nextline is assigned the read from <FH> it gets the second line, and the filehandle will then point to the 3rd line, and so on and so forth.
HTH

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broquaint