I fail to see what's so Perl specific about this question. The answer is 'Yes', but that has absolutely nothing to do with Perl. The answer would be the same, regardless of the language you write your web stuff in.

Only thing that matters is how HTTP works. The browser issues a request to an HTTP server. The server then responds, usually by sending the object. What happens between the arrival of the request and the sending of the response is up the server. You can do anything you like - in any language you like.