I assume you are using backtick or system(). If so the Windows command processor needs double quotes around the strings with spaces, and don't forget to deref the '\'.

Sample:

#!/usr/bin/perl
use strict ;

my $test3 = `dir \"C:\\Program files\"` ;

print ("Test: $test3\n") ;
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"Nothing is sure but death and taxes" I say combine the two and its death to all taxes!