I now have:

#! C:\perl\bin\perl.exe -w

use DBI;
#use strict;

my $DSN='Database';
my $driver;
my $servlist;

#connect to database
my $dbh=DBI->connect("dbi:ODBC:$DSN", "admin", "") || die;
my $sqlstatement=("UPDATE Servers SET Servers.nic1_driver_version=? WH
+ERE server=?");
my $sth=$dbh->prepare($sqlstatement);

#Open file for reading
open(INPUT,"file.txt") || die;
while (<INPUT>)
    {
    chomp;
    my ($servlist, $driver)=split(/,/);
    $sth->execute ($driver,$servlist) || die;
    }
close (INPUT);
$dbh->disconnect;
[download]

but still get:

DBD::ODBC::st execute failed: Microsoft ODBC Microsoft Access Driver Too few parameters. Expected 3. (SQL-07002)(DBD: st_execute/SQLExecute err=-1) at C:\Perl\scripts\test\newer.pl line 21, <INPUT> line 1.
Died at C:\Perl\scripts\test\newer.pl line 21, <INPUT> line 1.

Thanks again,
jw