To unapply (which doesn't seem to be supported in Moose nor Moo at the moment), presuming you have access to the original roles applied:

• find the superclass of the object (this will be the original class of the object without the roles applied)
• subtract the roles to unapply from the list of roles applied
• re-bless the object into its original class (in Moo, this is only necessary if the roles list is empty, as you use the class name in the next step)
• if the roles list isn't empty, create a new role application with the object's original class and new list of roles,
  bless the object into this new class (in Moose, apply roles)

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