Hello perl monks; How can I get the code to read from a file? It keeps asking me to enter sequence at line 4 but I need it to read from a file .txt file on my desktop.

How can I incorporate this into a code? 

 

# Open a .txt file

 open(seq1, "<seq1.txt") or die $!;

 #Assign open files to arrays

 my @seq1 = <seq1>;
 print "@seq1";

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#!/usr/bin/env perl

use warnings;
use strict;

# Open a .txt file

 open(seq1, "<seq1.txt") or die "Please enter two sequences as argumen
+ts on the command line\n"
    unless @ARGV == 2;

my ($orig, $comp) = map { uc } @ARGV;
die "Please enter only ATCG sequences\n" if grep { /[^ATCG]/ } $orig, 
+$comp;

die "Sorry, the two sequences you have just entered are of different l
+engths."
  . "\nPlease try again on the command line.\n" 
    if length $orig != length $comp;

# $comp will transform back to $orig iff it is the reverse complement
$comp =~ y/ATCG/TAGC/;

if ($orig eq reverse $comp) {
    print "Yes, the two sequences are reverse-complement of each other
+.\n";
    exit;
} else {
    die "Unfortunately, the two sequences are not reverse-complement.\
+n";
}

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In reply to How to read from a .txt by stamp1982

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