Bio::Tools::pICalculator:

"Calculates the isoelectric point of a protein, the pH at which there is no overall charge on the protein. Calculates the charge on a protein at a given pH. Can use built-in sets of pK values or custom pK sets."

See also Perl and Bioinformatics and pICalculator.

Update: For context, prior to OP updating their post it read "I have a fasta file containing many protein sequences in fasta format. I want to calculate isoelectric point of each sequences."

In reply to Re: Caluclation of isoelectric point of protein in fasta file using per; by marto
in thread Caluclation of isoelectric point of protein in fasta file using per; by yuvraj_ghaly

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