You're not quite reading my response right. What the OP wants is to retrieve the DNA sequence that corresponds to a specific ID. That's simple/fast enough, especially when using a hash. When I want to retrieve a subsequence, chr5:1234567-1234798 for example, which is only a portion of the sequence associated with a specific record in the FASTA file, then using Bio::DB::Fasta is far faster. The module has its uses, and is why someone implemented a similar thing in python as Pygr (the indexing approach, not the parser per say). You're not wrong, Bio::DB::Fasta is overkill for this specific purpose; I just don't see bashing a tool that has been helpful to bioinformaticists for something close to a decade. Also, it installs just fine on linux, where most of the users will be using it ;)

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