with PerlMonks I'm successfully created program for calculation of isoelectric point, molecular weight and amino acid types.
I'm thankful for all PerlMonks who helped me.
I now Seeking a major help in creating an interface for all these program where following specifications can be done:
(1) user can either paste their sequence or upload protein sequence in fasta format to calculate protein features discussed above.
(2) this interface can be incorporated into website.

In reply to Interface creation by yuvraj_ghaly

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