Hey guys! I'm new to Perl, and on my first assignment i've been developing a program to calculate the GC content of DNA sequences on a txt file. The file has +10 sequences and i'm only getting the result of the last one. Is there some problem with the loops ? The code is as follows. Thanks!

#!/usr/local/bin/perl

open (FASTAFILE, 'human_fold_25.txt');

$sequence = ' ';
$Ccount = 0;
$Gcount = 0;
$identifier = ' ';

while ($line = <FASTAFILE>) {
    chomp $line;
    
    if ( $line =~ /^>/ ) {
        $identifier = $line;
    } elsif ( $line =~ /^\./ ) {
        next;
    } elsif ( $line =~ /^\(/ ) {
    }else {
        $sequence = $line;
    }
}

$sequencelength = length ($sequence);

@nucleotides = split ( '' , $sequence );

foreach $nuc (@nucleotides) {
    if ( $nuc eq 'G') {
        $Gcount = $Gcount + 1;
    } elsif ( $nuc eq 'C') {
        $Ccount = $Ccount + 1;
    }
}

$GCcontent = ((( $Gcount + $Ccount )/ $sequencelength ) * 100);

close (FASTAFILE);

print "$identifier", ", ", "$GCcontent\n"

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In reply to Problem computing GC content by zuepinhel

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