Howdy zuepinhel, welcome to the Monastery!

Take a look at your initial while loop. You're assigning to $sequence (and $identifier) in each iteration, so when the loop finishes, $sequence will only contain the last line read, and all the rest of your script will only operate on that.

Try pulling the code following the loop into the loop -- except for closing the file, obviously.

In reply to Re: Problem computing GC content by AppleFritter
in thread Problem computing GC content by zuepinhel

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