Thanks Choroba for your reply.

So if I understood correctly, I should modify something like this:

if(    ($seq =~/^\w{2}A\w{6}T\w{2}[ACT]\w[^A{4}|^T{4}][GC]{4}[AT]/) 
   && ( $seq !~ /[GC]{9}/) 
   && ( $gcper >= 30 && $gcper <= 52) )

  { Do something....;}

[download]

Thanks again

In reply to Re^8: Filter and writing error log file by newtoperlprog
in thread Filter and writing error log file by newtoperlprog

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