I need to extract from a gbff:
gene complement(<5504..>6553) /locus_tag="OT_ostta02g00030" /old_locus_tag="Ot02g00030" /db_xref="GeneID:9832574"
the GeneID related to its coordinates $start = 5504 $stop = 6553 There are a lot of genes so I want to make a loop I use Perl for my code, you can see below a part of it (extracting the numbers) but it doesn't work.
$infile = "GCF.gbff"; $outfile = "a1.txt"; open(IN, $infile) or die "Failed to open $infile\n"; open (OUT, ">$outfile") or die "Failed to open $outfile\n"; $n=0; while ($line = <IN>) { chomp ($line); $line =~ s/\r$//; if ( $line =~ /<\d+..>\d+/ ) { ++$n; my ($start, $stop) = ($1, $2); print OUT "$start, $stop, $header"; } } print STDERR"$n"; sub header { @geneID = split /\s+/, $start, $stop; $header = "$fastaprompt$geneID[1]"; } close (IN); close (OUT);

In reply to gene location by nica

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