I need to extract from a gbff:
gene complement(<5504..>6553)
/locus_tag="OT_ostta02g00030"
/old_locus_tag="Ot02g00030"
/db_xref="GeneID:9832574"
the GeneID related to its coordinates
$start = 5504
$stop = 6553
There are a lot of genes so I want to make a loop
I use Perl for my code, you can see below a part of it (extracting the numbers) but it doesn't work.
$infile = "GCF.gbff";
$outfile = "a1.txt";
open(IN, $infile) or die "Failed to open $infile\n";
open (OUT, ">$outfile") or die "Failed to open $outfile\n";
$n=0;
while ($line = <IN>)
{
chomp ($line);
$line =~ s/\r$//;
if ( $line =~ /<\d+..>\d+/ )
{ ++$n; my ($start, $stop) = ($1, $2);
print OUT "$start, $stop, $header";
}
}
print STDERR"$n";
sub header
{
@geneID = split /\s+/, $start, $stop;
$header = "$fastaprompt$geneID[1]";
}
close (IN);
close (OUT);
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