$ENV{'PERL_TCL_DL_PATH'} = $pathTclDLL;

That line (from the "update" section of your post) looks dubious to me.
It sets: 
$ENV{'PERL_TCL_DL_PATH'} = $Bin . "\\tcltk2\\bin\\tcl86.dll";
but you probably want
$ENV{'PERL_TCL_DL_PATH'} = $Bin . "\\tcltk2\\bin";

[download]
I suspect that the updated script works because $ENV{PATH} provides everything that's needed, and that you could remove all lines that mention $pathTclDLL - and the script would still work.

Cheers,
Rob

In reply to Re: Link Tcl to a different tcl.dll on Windows by syphilis
in thread Link Tcl to a different tcl.dll on Windows by Takamoto

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