It seems that your out_file parameter is the same as your seq_file - so perhaps you're overwriting your input file while it is still processed. Try with a different output file.

In reply to Re: Use of uninitialized value in subroutine entry at /home/roarce/perl5/perlbrew/perls/perl-5.28.0/lib/site_perl/5.28.0/Seeder/Finder.pm line 476, <IN> line 8192. by haj
in thread Use of uninitialized value in subroutine entry at /home/roarce/perl5/perlbrew/perls/perl-5.28.0/lib/site_perl/5.28.0/Seeder/Finder.pm line 476, <IN> line 8192. by roarce92

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