You're welcome. And what is the goal of the script? To convert the DNA to ribose nucleic acid (RNA) ?
UPDATED CODE TO REFLECT TRANSLATING TO RNA
For starters, here is code I made (with acknowledgements to the Perl Monks for help) to parse a fasta file:
use strict;
use warnings;
use Data::Dumper;
my $file = '/path/to/your/file/here.fasta';
open (my $fh, '<', $file) or die "Could not open file '$file' $!";
my (%sequence_hash, $header);
while ( my $line = <$fh> ) {
chomp($line);
if ( $line =~ m/^>(.*)/ ) {
$header = $1;
}
else {
$sequence_hash{$header} .= $line;
}
}
# look at the header and sequence
print Dumper(\%sequence_hash);
my @translated_sequences;
foreach my $seq ( keys %sequence_hash ) {
$sequence_hash{$seq} =~ s/t/u/ig;
push @translated_sequences, $sequence_hash{$seq};
}
print Dumper(\@translated_sequences);
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