In that case, subtract 1 from the end value and you are good to go:
use strict;
use warnings;
my $seq =
'dddddddddBBBBBBBBBBBBDDDDDDDDBBBBBBBBBBBBBBddddddddddddddddddddBBBB
+BBBBBBBBBBDDBBBBBBBBddddddddddddd';
while ($seq =~ /(B+)/g) {
my $seg = $1;
my $seg_length = length ($seg);
my $seg_end = pos ($seq) - 1;
my $seg_start = $seg_end - $seg_length + 1;
print $seg. "|" . $seg_start . "-" . $seg_end . "\n";
}
$ perl 11153140.pl
BBBBBBBBBBBB|9-20
BBBBBBBBBBBBBB|29-42
BBBBBBBBBBBBBB|63-76
BBBBBBBB|79-86
$
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