First I downloaded all the est fasta sequences of tomato from NCBI.Then I submit that sequence file into an online tool EGassembler for sequence cleaning,vector masking,repeat masking,organelle masking and sequence assembly..As a result I got this contig file..This contig file is used for offline local blast..For doing blastn I downloaded the genome of Arabidopsis thaliana.Then I run the blast..

In reply to Re^2: how to create output file using perl by vineetha
in thread how to create output file using perl by vineetha

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