The main problem is in your regex declaration, you had / in there and it was being read as a regex and throwing errors about $regex not being initiated. as s//$regex///$subst//gi I think. Here is the error I was getting:

Use of uninitialized value $_ in pattern match (m//) at C:\User.....
[download]

Heres a solution I came up with:

use strict;
use warnings;

my $regex = '\{\[%tqu.*]}';
my $subst = '';

while(my $line = <DATA>) 
{
    $line =~ s/$regex/$subst/gi;
    print $line."\n";
}

__DATA__
1. Ka6 ({Of course not} 1. b6 $2 Kb7 $11) 1... Kb8 (1... f4 2. b6 $18)
+ {[%tqu "What is  White's next move?","","",g3,"",0,b6,"misses the wi
+n:",0]} 2. g3 $1 13. g6 c3
[download]

Outputs:

1. Ka6 ({Of course not} 1. b6 $2 Kb7 $11) 1... Kb8 (1... f4 2. b6 $18)
+  2. g3 $1 13. g6 c3
[download]

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