G'day reebee3,
Welcome to the Monastery.
I'd use substr to get the individual bases.
Then a simple comparison will identify your SNPs.
#!/usr/bin/env perl -l
use strict;
use warnings;
my ($seq1, $seq2) = qw{ATGGAT ACGGAG};
my $snp = 0;
for my $pos (1 .. length $seq1) {
my $base1 = substr $seq1, $pos - 1, 1;
my $base2 = substr $seq2, $pos - 1, 1;
next if $base1 eq $base2;
++$snp;
print "SNP $snp is the $base1 --> $base2 at position $pos";
}
Output:
SNP 1 is the T --> C at position 2
SNP 2 is the T --> G at position 6
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