Including -w produces the same result.

I've uploaded a small sample of my .txt file here:

https://www.dropbox.com/s/jelbmyf7nzn3n70/READS.txt?dl=0

In reply to Re^2: One-liner only checking the first line of the input file by TJCooper
in thread One-liner only checking the first line of the input file by TJCooper

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