HI, thanks for your time. The syntax error was for the line "open (IN2, "m2.gff") or die;" but as was pointed out I was missing a ; on the preceding line. Now I get the error message: Use of uninitialized value $columnsA2 in addition (+) at phastCons_multiples_ids3.pl line 19

In reply to Re^2: Error opening file handle within while loop by rjc33
in thread Error opening file handle within while loop by rjc33

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