Hi,
I'd would appreciate some help with formatting a fasta file with perl. My input file looks like this:
>dfbdbgf_356dfbdf
ATGGCTATCGATT
>sdgthhr_478364df
ATGGCTATCGATT
I want to have an output file that looks like this:
ATGGCTATCGATT 4
TGCATGCGCTACG 7
Basically, I want to:
-delete the header line and also delete reads that are NOT between 15-30 bp long
-Count the number of times that a read appears and put the value in column 2.
-Delete non-unique reads.
Thanks
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