Hello, Monks! Please, one more question. I didn't think I should start a new thread for this. 
while(
defined(my $head = <FASTQIN>) &&     
defined(my $seq = <FASTQIN>) &&      
defined(my $qhead = <FASTQIN>) &&    
defined(my $quality = <FASTQIN>)     
){
  substr($head, 0, 1, '>'); 
  my $temp = print $head, $seq;
}
  close (FASTQIN);  

my %count_seq;
    open my $fh, '<:encoding(UTF-8)', $temp or die "Cannot open $temp 
+$!";
    while (<$fh>) { 
        chomp;
        next if /^>/;                        
        next if length($_) > 30 or length($_) < 15; 
        $count_seq{$_}++;                  
    }

[download]
this is a section of code I am trying to write. Is there a way to make the variable  my $temp that was used in the while loop also available in the open filehandle statement? The code works OK till  close (FASTQIN);. the error reads: 
Use of uninitialized value $temp in open at test.pl line 44.
Use of uninitialized value $temp in concatenation (.) or string at tes
+t.pl line 44.
Cannot open  No such file or directory at test.pl line 44.

[download]
Line 44 is this: 
open my $fh, '<:encoding(UTF-8)', $temp or die "Cannot open $temp $!";

[download]
Kindly advise, monks! Thanks!

In reply to Re^2: Manipulating tab delimited file by andyBio
in thread Manipulating tab delimited file by andyBio

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