Group the peptides by frequencies, generate colours for the groups, not for individual peptides.
#!/usr/bin/perl
use warnings;
use strict;
use feature qw{ say };
use Color::Spectrum qw{ generate };
my %binders = ( HISCLTFGR => 16,
MQLFHLCLI => 8,
WGMDIDPYK => 4,
YVNVNMGLK => 3,
AYRPPNAPI => 2,
ELMNLATWV => 2,
EYLVSFGVW => 1,
FFPSIRDLL => 1,
FLPSDFFPS => 1,
IISCSCPTV => 1,
);
my %freq;
push @{ $freq{ $binders{$_} } }, $_ for keys %binders;
my @colours = generate(scalar keys %freq, '#FF0000', '#0060BF');
my %colour;
@colour{ @{ $freq{$_} } } = (pop @colours) x @{ $freq{$_} }
for sort { $a <=> $b } keys %freq;
say "$_ : $colour{$_}" for keys %colour;
($q=q:Sq=~/;[c](.)(.)/;chr(-||-|5+lengthSq)`"S|oS2"`map{chr |+ord
}map{substrSq`S_+|`|}3E|-|`7**2-3:)=~y+S|`+$1,++print+eval$q,q,a,
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