This is because you're reading the entire file into an array. To reduce the memory footprint, you're better off reading the file a line at a time.
Can you please show us an example of the line you're doing this on?:
my $index = first{$headers[$_] eq 'Strand'} 0..$#headers;
We should be able to help you rewrite your code if we know where $index is being gleaned from.
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