Hello, I have a script which hashes the sequences of a pretty hefty file whereby the hash is the DNA sequence and the key is the sequence ID. I am now trying to cycle through my hashes using a foreach loop in an attempt to sift out sequences which have a specific length. This length will be set using a variable for minimum and maximum length at the user's discretion. I am having problems trying to accomplish this task though. I'm not sure how to return the character length of the hash. In order to pick my desired sequences. My script so far is as follows:
open F, "human_hg19_circRNAs_putative_spliced_sequence.fa", or die $!; my %seq = (); my $id = ''; while (<F>){ chomp; if ($_ =~ /^>(.+)/){ $_ = $1; }else{ $seq{$id} .= $_; } } my @seqarray; my $maxlength = 150; my $minlength = 100; foreach $id (keys %seq){ if ((length$seq{$id} <= $maxlength) || (length$seq{$id} >= $minlen +gth)){ @seqarray = ($id."\n",$seq{$id}); } } print join ("\n", @seqarray); close F;

In reply to Picking out specific lengths from a set of hashes. by Peter Keystrokes

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