Hello pdahal,

As the fellow monk kcott asked you for Short, Self-Contained, Correct Example, I would suggest to try to understand why he did? It is not because he wants to torture you, or for any other reason than simply it is necessary for us to be able to reproduce your problem. Most of the monks here are not expert on all areas but are willing to test and experiment with different areas.

If you do not provide us with anything how can you expect anybody to help you?

Having said that I went online and I did a random search using your keywords and the first result that I found is Access abstract from pubmed using Bio::DB::EUtilities. I have no idea if this is something that you are looking for or you want to use another module other than Bio::DB::EUtilities.

According to the comments the full example provided by the developers is working (I have not tested it).

On your next question it would be better for you to come a bit prepared with more information. We can not read your mind and understand what you want to do or how you want to do. Since everyone is devoting their free time to help you I think it would be better to provide us much information as possible.

Update: I could not resist to give it a try, so the following piece of code it works (taken from the link that I provided you):

#!/usr/bin/perl use say; use utf8; use strict; use warnings; use XML::LibXML; use Bio::DB::EUtilities; my @ids = (23298400); my $factory = Bio::DB::EUtilities->new( -eutil => 'efetch', -email => 'mymail@foo.bar', -db => 'pubmed', -retmode => 'xml', -id => \@ids); my $xml = $factory->get_Response->content; my $xml_parser = XML::LibXML->new(); my $dom = $xml_parser->parse_string($xml); my $root = $dom->documentElement(); for my $node ($root->findnodes('//*[text()]')) { my $name = $node->nodeName(); if ($name eq 'Abstract') { for my $child ($node->findnodes('*')) { binmode STDOUT, ":utf8"; say $child->textContent(); } } } __END__ Adult cardiomyocytes have little ability to regenerate, thus cardiac r +egeneration therapy represents a potential method for treating severe + heart failure. Human amniotic mesenchymal cells (hAMCs) have the pot +ential to be a useful cell source for cardiac regeneration therapy. W +e attempted to isolate stem cells from hAMCs and differentiate them i +nto cardiomyocytes. Nanog promoter-Cre plasmid and cytomegalovirus (C +MV) promoter-loxP-STOP-loxP-Red-puro(r) plasmid were co-transfected i +nto immortalized hAMCs (iHAMs). Nanog-positive iHAMs were treated wit +h 5-azacytidine (5-aza), trichostatin A (TA), activin A (AA), and bon +e morphogenetic protein-4 (BMP-4), or co-cultured with murine fetal c +ardiomyocytes for cardiomyocytes differentiation. Isolated Nanog-posi +tive iHAMs were analyzed by quantitative RT-PCR and immunofluorescent + staining before and after differentiation. Expression of Nanog, Oct3 +/4, Sox2, and Klf4 was significantly higher in Nanog-positive than in + Nanog-negative iHAMs. Nanog-positive iHAMs were stained for Nanog an +d Oct3/4 in the nucleus. Nanog-positive iHAMs treated with 5-aza expr +essed Nkx2.5, GATA-4, human atrial natriuretic peptide (hANP), cardia +c troponin T (cTnT), myocin light chain (Mlc)-2a, Mlc-2v, β-myos +in heavy chain (β-MHC), hyperpolarization-activated cyclic nucle +otide gated channels (HCN)-4, and inwardly rectifying potassium chann +els (Kir)-2.1. Although Nanog-positive iHAMs treated with TA, AA, or +BMP-4 expressed several cardiac markers, no contraction was observed. + Co-cultured Nanog-positive iHAMs with murine fetal cardiomyocytes sp +ontaneously contracted in a synchronized manner and expressed the car +diac markers. In conclusion, Nanog-positive hAMCs with characteristic +s of stem cells were isolated and differentiated into cardiomyocyte-l +ike cells, suggesting that these isolated hAMCs could be a useful cel +l source for cardiac regeneration therapy.

Is this something that you are looking for

Hope this helps.

Seeking for Perl wisdom...on the process of learning...not there...yet!

In reply to Re: Forward tracing a text by thanos1983
in thread Forward tracing a text by pdahal

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