Hello Nicpetbio23!,

The following example uses a couple CPAN modules (Path::Tiny, and Capture::Tiny). The syntax of your tblast command does not seem to be quite right. Detailed descriptions for tblastn command line arguments can be obtained with tblastn -help. I modified your tblastn command based on my reading of the help output.

This code assumes that there are fasta query files in the current directory with .fa file extensions. For each fasta file found, an output file will be generated (with a .txt extension).

NOTE: I did not test this code thoroughly. I commented out the call to system to be sure that the intended $cmd string is generated, but I did not run the tblastn commands.

#!/usr/bin/env perl

use strict;
use warnings;

use Path::Tiny;
use Capture::Tiny ':all';

my @paths = path('./')->children( qr/\.fa$/ );

foreach my $file_path (@paths){

    my $query_file = $file_path->stringify;

    my $output_file = path(
        $file_path->parent,
        $file_path->basename( '.fa' ).'_output.txt'
    )->stringify;

    my $cmd = "tblastn -query $query_file -db genome.db".
              " -out $output_file";

    print "Query file: $file_path\n";
    print "\t $cmd\n";

    my ($stdout, $stderr, $exit) = capture {
        system( $cmd ); 
    };

    if ($exit != 0){
        die "Error: command failed";
    }
}

exit;

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In reply to Re: tblastn unix by kevbot
in thread tblastn unix by Nicpetbio23!

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