Indeed, I found that to be the main issue when trying to think of a way to do this. I could definitely split the FASTA file beforehand, although I try to minimise the amount of steps required to run a script hence the question! Thanks for your input.

In reply to Re^2: Loading only portions of a file at any given time by TJCooper
in thread Loading only portions of a file at any given time by TJCooper

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