Thank you monks. I have tried any possible solution (cleaning the path for Windows, setting ENV etc.), but NO solution works in the packed versions (pp). And I really do not understand why! Here is a better script that reproduces the problem (the first part works, the second no).

use warnings;
use strict;
use DBI;
use DBD::SQLite;

#Working part with hard coded path    
my $dbfile1="C:/users/DON/Desktop/data.db";
print "\nFIRST Connection to dbfile path read from script ($dbfile1) .
+.. ";
my $dbh = DBI->connect("dbi:SQLite:dbname=$dbfile1","","") || die $DBI
+::errstr;
print "done!\n\n";
    
#NOT working part with path from file    
my $dbfile2;
print "Reading dbfile path from configuration file... ";
my $FileInput="config.txt";#simple txt file containing this: C:/users/
+DON/Desktop/data.db
open my $FH, "<:encoding(UTF-8)", $FileInput || die (print "can't open
+ file $dbfile2");
while (my $line = <$FH>) {
    $dbfile2 = $line;
    chomp $dbfile2;
}
print "$dbfile2\n";

print "SECOND Connection with dbfile read from file $dbfile2 ... ";
$dbh = DBI->connect("dbi:SQLite:dbname=$dbfile2","","")|| die $DBI::er
+rstr;
print "done!\n";
[download]

Again, from the command line the script works correctly. The by pp compiled version behaves like this:

FIRST Connection to dbfile path read from script (C:/users/DON/Desktop
+/data.db) ... done!

Reading dbfile path from configuration file... C:/users/DON/Desktop/da
+ta.db
Can't connect to data source 'dbi:SQLite:dbname=C:/users/DON/Desktop/d
+ata.db' because I can't work out what driver to use (it doesn't seem 
+to contain a 'dbi:driver:' prefix and the DBI_DRIVER env var is not s
+et) at script/myscript.pl line 22.
SECOND Connection with dbfile read from file C:/users/DON/Desktop/data
+.db ...
[download]

I

I've been stuck for days on this problem. So any help is very much appreciated

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