Thank you @ tybalt89. This should work. As I mentioned in my original post, my scripting is very rusty after nearly a 4 year gap. I understand only bits and pieces of your code.

One guess is that your script requires IDs to end in numbers, which you rely on to process and split the input data? Yes, no , may be? I'd prefer the distinction between ID line and sequence line be based on whether it is preceded by ">" symbol or not, for ID and sequence, respectively, please. How should i modify the script for that?

It appears to me that the script s counting A/T/G/C for each sequence individually, correct? If not, please skip. But if yes, then how much more RAM-hungry would a modification be where the A/T/G/C count frequency across ALL sequences are first calculated BEFORE generating sequences that match those frequenciues?

Finally, how do I accept input through a FH and output to a new FH. I tried several mods to your script, but only a few worked out. Hence this request for your additional assistance. Thanks a ton!

In reply to Re^2: Reduce RAM required by onlyIDleft
in thread Reduce RAM required by onlyIDleft

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