You probably should use special function described in Win32-SDK help files.
Most probably function in interest is DeviceIoControl. I see some words about locking a volume here, like FSCTL_LOCK_VOLUME.
You can use those functions via Win32::API module.

Best wishes,
Vadim.

In reply to Re: Perventing eject of zip disk on windows by svad
in thread Perventing eject of zip disk on windows by rbc

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