Hi,
Firstly i'd like to thank everyone that took the time to help me
yesterday. I now have another slight problem....on the same lines.
Anyway, i am trying to align two short sequences of DNA and mark on mismatches, which i have done. Yesterday i got help trying to interleave and wrap the
output to print 60 chars per line.. my problem is now that i need to interleave the mismatch markings aswell, this part isn't working.
the snippet of code that i have is below;
my $mismatches = get_mismatches ($genome1, $genome2);
my @strings = ($genome1, $mismatches, $genome2);
for (my $x = 0; $x < length ($strings [0]); $x +=60) {
print join ("\n", map (substr ($strings[$_], $x, 60), 0..2 )). "\n\n
+";
}
sub get_mismatches {
($genome1, $genome2) = @_;
my ($length) = length ($genome1);
my $position;
my $count = 0;
print "\n\n";
for ($position = 0; $position < $length; ++$position) {
if (substr($genome1, $position, 1) eq substr ($genome2, $position
+, 1))
{
print "-";
}
else {
print "*";
}
}
return ($genome1, $genome2);
}
The desired output would look like this;
ACACTTGCATACTGATC
--*****-****---**
ACTACGACTGCATGACT
TGACTGCACT
*********-
CATGATATGT
but so far it looks like this;
ATGCATGACTACT
CTGAATGCTGACT
CGTCTGACTATAA
ATGACTTGAC
CTATGACTGA
CCGATGACTG
so for some reason it thinks that $mismatches is a string of DNA
nOt the combination of * and - that were in the subroutine.
If anyone can help i'd really appreciate it.
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