If all you wanted to do was grab the middle number on each line, this worked for me:
while(<DATA>) {
if (m/\w{1,12}\s+(\d{1,5})\s+[a-zA-Z]{4}/) {
my $site = $1;
print "$site\n";
}
}
__DATA__
BC001593 91 NPSL
BC001593 260 NASS
BC001593 293 NAST
# the output
91
263
293
As far as performing this regex until there are no more lines, I think it depends on where the protein strings are stored. Either way, if you can get the lines in to an array, you could use the foreach construct to touch every one. HTH
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