I'm the guy who started this thread, and I wanted to follow up with some code that I wrote, and ask other monks if this is ok. Again, thanks in advance.
sub blast
{
my $data = shift;
my $temp = "$ENV{HOME}/temp/seq.tmp";
my $fifo = "ssh myhost /path/to/blast";
my @args = ('-p blastn', '-d nt', '-e 0.0000001', '-m 7');
### load input file with fasta-formatted queryid/sequence ###
open SEQ, ">$temp" or die "Could not open for write:$!";
print SEQ ">",$data->[0],"\n",$data->[1];
close SEQ;
### open a handle to BLAST output, retry if unsuccessfull ###
{
if (!open BLAST, "$fifo @args <$temp|")
{
#
print LOG "BLAST connection refused, retrying...\n";
#
sleep(300);
redo;
}
}
### process the report ###
parse(*BLAST);
}
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