You don't understand how sort works.
You should know that sort expects a list (an array) to sort. Not just one element. When you call a subroutine to "sort everything in $start_bit_no", you will sort a scalar, i.e. JUST ONE NUMBER (or string).
And you made a mistake in your assignments:
$start_bit_no = @column1[3]; #don't use this when you mean
$start_bit_no = $column1[3]; #this
I suggest that you show us the whole piece and not just some parts to let us guess the rest.
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