Fantastic - thanks! Unfortunately, I can follow neither of your two above suggestions, although don't think I haven't contemplated it. Unfortunately a variety of the species involved have multiple color morphs, or are exactly the same color as another species (I mean you have to zoom in on individual zooids in a colony to tell the difference- hence we're using a high-res digital underwater camera).

Saving different layers in a PSD would be nice, but the problem there is that often we have species overgrowing each other in a non-lethal way (e.g. a colonial sea squirt overgrows a mussel. Both live, and both need to be counted). The current scheme I'm going with is copying the area of each species, putting it into its own .gif, and using some filename information for the final data file.

Although if you know a way to do all of that in GIMP, I'd love to hear it!

In reply to Re: Re: pixel counting on a fouling panel by redbeard
in thread pixel counting by redbeard

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