While you're fixing up spelling mistakes, "struckt subField"? "struck Field"?
Have you considered verifying whether $tree->{level1}->{level2}->{$item} and $tree->{level1}->{level2}->{$item}->{subItem} exist, before accessing them?
If $self is a reference to a hash, what is $self->$item, $self->$item->$subItem? A subroutine, I guess, since it's invoked and passed $tree->{level1}->{level2}->{$item}->{subItem}.
--
TTTATCGGTCGTTATATAGATGTTTGCA
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