You have to create the table first. I don't think DBD::SQLite supports some syntax like LOAD DATA LOCAL INFILE 'blah.txt' INTO TABLE foo like MySQL and friends. All you need is something like (untested):

use DBI;

my $file = "C:/test.txt";

my $dbh = DBI->connect("dbi:SQLite:dbname=dbfile","","");

 create the table to suit
my $sql =<<SQL;
CREATE TABLE stuff (
    id INT,
    field CHAR(32),
    PRIMARY KEY(id) )
SQL


$dbh->do( $sql ) or die "Can't create table\n" . $DBI::errstr;

 need as many ? bind values as fields
my $sth = $dbh->prepare( "INSERT INTO stuff VALUES(?,?)");

open TXT, $file or die $!;
while(<TXT>) {
    chomp;
    my ( $id, $field ) = split "\t";   # or whatever delim
    $sth->execute( $id, $field );
}
close TXT;
$sth->finish;
$dbh->disconnect;
print "Done!\n";
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