Perl doesn't know you mean to pass a filehandle to that method, so it passes the bareword "TR" instead. You could try to pass \*TR instead. 
$ftp->put(\*TR,$File);

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Better yet, for a recent enough perl, use a lexical variable to hold the filehandle. It'll look cleaner. 
open my($tr), "unix2dos $File |" or die $!; # Or FQ path
$ftp->binary(); # We translate, not the server.
$ftp->put($tr,$File); # must give remote name.

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But, IMO, it's overkill to call an external program to handle this little task. Perl will convert line endings for you, when reading from a handle in text mode. Internally, on Windows, text data is Unix text data. So, all you need to do is open the file. 

open my($fh), $File or die $!;
$ftp->binary(); # We translate, not the server.
$ftp->put($fh,$File); # must give remote name.

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p.s. I really wonder why just transferring data in Ascii mode won't work. Are you sure you don't accidently have double carriage returns in your files?

In reply to Re^3: convert files unix2dos using perl script by bart
in thread convert files unix2dos using perl script by cc

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