Then open the file within the for-loop:

#!/usr/bin/perl -w
use strict;
use Bio::SeqIO;

my $file = $ARGV[0];

for (my $trial = 1; $trial <=2; $trial++)
{
   open INFILE, "<$file" or die "$0:  Can't open file $file: $!";
   seek(INFILE,0,0); #This is line 10
   print "Trial $trial\n";

   my $i =1;
   my $in = Bio::SeqIO->new(-format => 'fasta', -fh => \*INFILE);

   while( my $seq = $in->next_seq  ) {
      print $i++, " : ", $seq->seq(), "\n";
   }

}

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In reply to Re^3: How to avoid using array in concatenating string of multiple lines by reneeb
in thread How to avoid using array in concatenating string of multiple lines OR How To Read FASTA by monkfan

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