$targetseq =~ /.*$probe.*/
simplies to
$targetseq =~ /$probe/
but you probably want to escape the stuff in $probe (which should fix the problem), leaving you with
$targetseq =~ /\Q$probe\E/
which is the same thing as
index($targetseq, $probe) != -1
So
if ($targetseq=~ /.*$probe.*/) {
$start = index($targetseq, $probe);
push(@matchregion,$start);
}
becomes
$start = index($targetseq, $probe);
push(@matchregion, $start) unless $start == -1;
I didn't look at improving your alogirthm, just fixing the problem you mentioned (the contents of $probe being treated as a regexp).
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